Effect of treatment with fruits extracts on some biochemical and antioxidant parameters in rats

Abstract

Reactive oxygen species (ROS) have high potential to damage almost all types of cellular constituents, which explains their involvement in the induction a number of pathologies. The supplementation of the body by exogenous antioxidants seems to be very helpful to fight these harmful species.Fruits are an important source of exogenous antioxidant which can minimize oxidative damage. The aim of this studyis the evaluationpolyphenols, flavonoids, tannin, sugars and protein contents, the identification of some phenolic compounds, the antioxidant and hepatotoxicity activity of methanolic extracts of the several fruits consumed in Algeria.The identification of phenolic compounds by UPLC and HPLC/MS showed deference between fruits extracts. Gallic acid is the major compounds present in all fruits extracts. The results revealed that Fargariaananassacontains high amount of polyphenols, flavonoids and protein. The high level of tannin was found in Punicagranatum.While, the high level of sugars was found in Ficus.The antioxidant activity was evaluated in vitro using the DPPH, ABTS, hydroxyl radicals,reducing power, ferrous ion chelating tests, β-carotene/ linoleic acid model system, ferric thiocyanate method and thiobarburic acid method. Strawberries extracts were the best one can scavengers of free radicals.However Ficusextracts was the better chelator compared to other fruits. Fargariacan inhibit lipid peroxidation by deferent ways. The antioxidant activity of extracts at doses of 200 and 600 mg/kgin vivowas assessed in rats for 14 days, on plasma antioxidant capacity (PAC), oxidative stress markers in liver and kidney and on some biochemical parameters. Results showed that all extracts at 600 mg/kg were effectives against stress oxidative. The hepatoprotective effect of fruits extracted was evaluated in rats intoxicated by CCl4. Results showed that CCl4induced remarkable hepatotoxicity. All fruits extracts have an important protective effect and reduce hepatic and renal tissues damage induced by this toxic agent. The administration of 200 and 600 mg/kg of Ficuscaricashowed a significant hepatoprotective effect, it improve the oxidative stress markers (GSH and MDA) and hepatic biochemical parameters and this results confirmed by histological examination. In conclusion, Fruit extracts examined in this study showed a very high antioxidant activity and hepatoprotective properties which wererelated to their contents in polyphenols and flavonoids