Etude phytochimique, caryologique et activités biologiques des huiles essentielles du genre Helichrysum Auct. Plur. de l'est Algérien

Abstract

Extraction of the aerial parts of the essential oils of Helichrysum italicum var numidicum and H. lactuem var typicum and two subspecies, H. stoechas ssp scandens and H. stoechas ssp rupstre, was carried out by hydro distillation. The extraction gave a viscous liquid with a low yield with an average of 0.025%. The chemical composition of the essential oils of the genus Helichrysum was analyzed by GC and GC / MS. The analysis identified 46 compounds with the presence of significant interspecific variability. Statistical analysis confirms the separation of the genus Helichrysum species with the presence of chemical variability between these species. Evaluation of the antimicrobial activity of Helichrysum essential oils has shown a modest effect against all bacteria tested, while they show no significant antifungal activity against Candida albicans. Evaluation of the cytotoxic activity of the essential oils of the species of the genus Helichrysum was carried out on a line of breast cancer cells. The results obtained showed that the essential oils of the species have no cytotoxic activity on MCF7 cancer cells. The karyology study of the genus Helichrysum made it possible to determine a tetraploid chromosomal number 2n = 4x = 28 with a base number x = 7 for each of its species.