Cellular engineering Intended for first-year masters students Biological sciences Biotechnology and health speciality

Abstract

This handout is the written support for the module “Cellular Engineering” intended for students of the Master 1 specialty biotechnology and health. The courses in this module aim to acquire the theoretical and practical foundations of cell engineering applied discipline which is devoted to the modification of genetic sequences in living cells, It brings together in a condensed way the essential information on the methods of studying cells and molecules as well as the most used analytical techniques on the cell in quantitative and qualitative analysis. This document is designed according to the official outline prescribed by the Ministry of Higher Education and Scientific Research. These courses are organized in seven chapters: 1. First, the fractionation techniques are presented. In addition, the different principles are discussed and the materials used; 2. In the second chapters, immunomarking techniques were presented. 3. The third chapter is devoted to cell observation techniques: optical and electron microscopy, their principles, types, sample preparation and their domains of application. 4. The fourth chapter presents the flow cytometry methods, Tricellular by quoting the different types, their principles and the materials used. 5. The fifth chapter presents cell cultures: types, conservation methods and experimental conditions. 6. The sixth chapter deals with toxicity and viability tests performed on cells, their advantages and disadvantages 7. The last chapter summarizes the cell, tissue and animal models while discussing the types used, appropriate choices and ethical laws.