Identification des enzymes clés impliquées dans la biodégradation de la matière organique de Bacillus sp2 et Pseudomonas sp2

Abstract

In this work, two bacterial strains, Bacillus sp2 and Pseudomonas sp2, isolated from soil water samples, were evaluated for their ability to produce extracellular enzymes of biotechnological interest. Enzymatic activities were detected by qualitative tests on agar. Bacillus sp2 showed high amylase activity with an average diameter of (1.85 cm), strong protease activity with an average diameter of (2.1 cm), particularly high cellulolytic activity with an average diameter of (3.2 cm) and an absence of chitinolytic activity. On the other hand, Pseudomonas sp2 showed low amylase activity with an average diameter of (0.4 cm), protease activity (1 cm), low cellulolytic activity (0.3 cm) and moderate chitinolytic activity of (0.75 cm). These results indicate a complementary enzymatic profile between the two strains, suggesting their potential in various industrial applications such as biomass degradation, organic waste treatment and enzymatic valorization