ANTI-OXIDANT AND ANTI-INFLAMMATORY EFFECTS OF PLANT METABOLITES

Abstract

The aim of this study was to determine the anti-inflammatory and antioxidant activity of flavonoids (genistein, gossypin, myricetin, silymarin, diosmin, naringin, Quercetin,), phenolic acids (caffeic acid, gallic acid) and other plant metabolites (astaxanthin and vitamin C) on AAPH- induced hemolysis, myeloperoxidase and elastase activities. The in vitro antioxidant activity of flavonoids and other plant metabolites was evaluated and exerted a significant protective effect against AAPH-induced erythrocyte hemolysis, with a good correlation between concentration and half-hemolysis time (HT50). The plant metabolite that exhibited the highest anti-hemolytic effect was caffeic acid with half- hemolysis times of 3.44 and 2.62 hours at 5and 1 μg /ml respectively silymarin and astaxanthin had the lowest effect in comparison with the other plant metabolite. The study of plant metabolites showed also significant inhibitory effects on elastase and MPO activities. The anti-inflammatory activity was confirmed by evaluating the effects of plant metabolites on inhibition of elastase activity and neutrophil myeloperoxidase activity. The plant metabolites with the highest inhibition effect on MPO activity was caffeic acid with inhibition percentage of 93.65% and 59.6% at 5 and 1 μg /ml) while astaxanthin had the lowest effect with 4.72% and -14.15% at concentrations 5 and 1 µ𝑔/??. The plant metabolites also inhibited neutrophil elastase activity with vitamin C exhibiting the highest inhibition effect of 24.75% and 23% at 10 and 5 µ𝑔/?? while silymarin had the lowest inhibition effect of 0% at 10 𝜇𝑔 /ml and 6.25% at 5 𝜇𝑔 /ml. The obtained results showed that plant metabolites have considerable antihemolytic, antioxidant, and anti- inflammatory effects.