Isolement de nouveaux dérivés de la carboxyméthylcellulose sodique : étude chimique, couplage avec des biopolymères azotés et élucidation des comportements biologiques

Abstract

The work done in the context of our LMD PhD thesis in Pharmaceutical Engineering aims to study the chemical modification of carboxymethylcellulose by two methods. The first concerns the acidification of CMC and the second is devoted to the oxidation reaction of this polysaccharide in presence of sodium hypochlorite as oxidizing agent and TEMPO/NaBr as a reaction catalyst. Hydroxylamine and acid-base assay methods were used to quantify the aldehyde and carboxyl groups, respectively. Chitosan was then coupled with CMC and oxidized CMC. Free amine groups carried by chitosan were estimated by potentiometry and conductimetry. Characterization and identification of hydrogels thus obtained are made by IR-TF, FRX and ATG / DSC. The morphology was examined by opacity test. However, the biological behaviors of new biomaterials have been investigated by different studies such as biocompatibility with human blood; all our compounds are non-hemolytic. The anti-inflammatory activity performed by a preliminary test on egg albumin also indicates that our components are devices with inhibitory ability to denature proteins. Mucoadhesive tests show a marked increase in the adhesion times of HCMC, CMC / CS and OCMC / CS on the intestinal mucosa compared to CS and CMC. Finally, the results of the biodegradation tests show that our hydrogels are biodegradable and can be removed from human fluids over a relatively short period of time. The success in the preparation of Doxycycline and Levomepromazine transdermal patches based on CMC / Gel / PVA or OCMC / Gel / PVA was confirmed by the various mechanical and biological tests undertaken during our studies.