Évaluation des activités antioxydante, anti-inflammatoire et antimicrobienne des extraits de delphinium.

Abstract

Medicinal and aromatic plants have been used in traditional medicine since antiquity as a source of seasoning or as a remedy. The objective of this study is to determine the content of total phenolic compounds and flavonoids of two extracts (aqueous and hydro-ethanolic) of the aerial part of Oriental Delphinium, and to test their antioxidant, anti-inflammatory and antimicrobial properties. The polyphenol content was determined by the Folin-Ciocalteu method and the flavonoid content was determined by the aluminum trichloride method. Antioxidant activity was assessed by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical test. The extracts showed a content of total phenolic compounds of 3.39 ± 0.176 and 3.586 ± 0.22 µg EAG/mg extract for the aqueous and hydro-ethanolic extract, respectively. A flavonoid content of 7.826 ± 0.208 and 9.812 ± 0.303 µg EQ /mg of extract, respectively. And a value of CI50: 128.22 ± 0.07 and 188 ± 13.97 µg/ml comparable to the effect of BHT with CI50: 6.29 ± 1.12 µg/ml for the DPPH test of the two aqueous and hydro-ethanolic extracts, respectively. For the anti-inflammatory test, the results showed that at low concentrations the antiinflammatory activity is average compared to diclofenac. The antimicrobial activity was evaluated for 04 bacterial strains (Gram-: Eschericha Coli ATCC 25922, Pseudomonas Aeruginosa ATCC 27853, and Gram+: Staphylococcus Aureus ATCC 25923, Bacillus Subtilis ATCC 6633), by the then diffusion method. The result was very low, almost nil for the two aqueous and hydro-ethanolic extracts.