Activités antioxydantes et anticoagulantes des extraits des polyphénols du Cratægus Azarolus L.
Loading...
Date
Journal Title
Journal ISSN
Volume Title
Publisher
Université Sétif 1 Ferhat Abbas . Faculté des sciences de la nature et de la vie
Abstract
Description
The aim of this study was to evaluate the antioxidant and anticoagulant activity after
phytochemical screening and quantification of bioactive compounds, notably polyphenols and
flavonoids, of three phenolic extracts (aqueous, hydro-ethanolic and hydro-methanolic) from
Crataegus Azarolus leaves harvested in the Beni-Aziz region, wilaya of Sétif. Extraction,
carried out by ultrasound after Soxhlet delipidation, showed the yields of 10.11 %, 20.37 % .
The highest total polyphenol content was recorded for the methanolic extract, followed by the
aqueous extract and finally the ethanolic extract, which yielded a level close to that of water.
As for flavonoids, the highest content was obtained with the aqueous extract, followed by the
hydro-ethanolic and hydro-methanolic extracts. Phytochemical screening revealed the presence
of tannins, coumarins and reducing sugars in all three extracts. On the other hand, saponins and
anthocyanins were present only in the two alcoholic extracts and absent in the aqueous extract.
Alkaloids and starch were totally absent from these extracts. Antioxidant activity was assessed
using two tests : DPPH method and Phenanthroline method. The results indicate that all three
extracts have good free radical scavenging activity against DPPH. The aqueous extract was the
most active with an IC50 of 87.22 µg/ml, followed by the hydro-ethanolic extract (IC50 =
365.37 µg/ml) and the hydro-methanolic extract (IC50 = 559.15 µg/ml). The results of the
Phenanthroline method show that all the extracts are capable of reducing iron and forming a
Fe2+phenanthroline complex, with the hydro-ethanolic extract showing the best iron reduction
activity. The anticoagulant activity of the phenolic extracts was also assessed in vitro, the results
indicate that all three extracts have a relatively high dose-dependent anticoagulant activity.