Modulation of oxidative stress and hemostasis by methanolic extract of Coriandrum sativum seeds.

Abstract

This study aimed to evaluate the antioxidant, anticoagulant, and thrombolytic activities of the aqueous-methanolic extract of coriander seeds using in vitro experimental methods. Phytochemical analysis revealed that the extract contains significant levels of phenolic compounds with total phenolic content of 69.87 ± 5.62 µg GAE/mg and total flavonoid content of 26.54 ± 1.45 µg RE/mg. The DPPH assay demonstrated a notable free radical scavenging capacity with an IC₅₀ of 80 ± 5.13 µg/ml. Similarly, the total antioxidant capacity (TAC) test showed an A0,5 of 39.33 ± 4.62 µg/ml, indicating considerable antioxidant potential. Hemolysis inhibition assay revealed a protective effect of the extract on red blood cell membranes, with an IC₅₀ of 0.244 mg/ml, suggesting its role in mitigating oxidative damage. Regarding anticoagulant activity, the extract significantly prolonged clotting times, with PT and aPTT values reaching approximately 57.66 and 68.66 seconds, respectively, at the highest concentration tested (75 mg/ml), indicating interference with both intrinsic and extrinsic coagulation pathways. In thrombolytic assay, fibrinolytic activity increased from 15.82% at 3.125 mg/ml to 35.96% at 50 mg/ml, supporting the extract’s clot-dissolving potential. These findings highlight Coriandrum sativum as a promising natural source for the development of effective therapeutic alternatives targeting oxidative stress, coagulation disorders, and thrombotic conditions, with potential applications in preventive and complementary medicine.