’mpacti Des Conditions Pre-Analytiques Sur La Stabilite Des Parametres De L’hemogramme

Abstract

The complete blood count (CBC) is an essential laboratory test in clinical practice, and its reliability depends on the proper control of pre-analytical factors. This study, conducted in the Hemobiology Unit of the Sétif University Hospital Center (CHU of Sétif), aimed to evaluate the impact of storage time and temperature on the stability of CBC parameters. Blood samples were stored at room temperature (23°C) and at 4°C and analyzed at different time intervals using the Celltac G+ hematology analyzer (Nihon Kohden). The results demonstrated good stability of red blood cells, hemoglobin, and platelet counts. However, several parameters, including mean corpuscular volume (MCV), red cell distribution width (RDW), mean platelet volume (MPV), lymphocytes, basophils, and reticulocytes, showed significant variations, particularly when samples were stored at room temperature. Storage at 4°C provided better preservation of most hematological parameters for up to 48 hours. These findings highlight the importance of appropriate sample storage conditions to ensure the reliability of hematological results and improve laboratory quality management.