Mise au Point d’une Méthode de Dosage de la Vancomycine par Chromatographie Liquide à haute Performance

Abstract

In order to be able to measure the serum levels of vancomycin in patients’ blood hospitalized in the CHU Sétif, who take daily cures of vancomycin 500mg/day (see more), the analytical chemistry laboratory of the faculty of pharmacy - Sétif- took the initiative to develop and then validate a method for assaying this ATB using the HPLC method. The development of an analytical method consists in optimizing its parameters: (pH; stationary phase; mobile phase; flow rate; injection volume, etc.), taking into consideration the physicochemical characteristics of the active ingredient to be assayed vancomycin. (Absorption wavelength; solubility ; solvents...etc.) The objective of our work is the development and optimization of an HPLC/DAD method, for the dosage of vancomycin according to the SFSTP recommendations. The effects of the composition in type and percentage of the mobile phase, flow rate, injection volume, and detection wavelength were all studied. Chromatographic method was performed on a C18 column (250mm×4.6mm, 5um) at room temperature with a mobile phase consisting of acetonitrile/phosphate buffer (14:86; v/v), at a flow rate of 1,5ml/min , detection at 200nm and an injection volume of 10ul. The elution of vancomycin was observed at 2.529 min with a tailing factor of 1.920 and theoretical plate of 4190.707.