Contribution à l’étude de l’activité biologique et la composition chimique des huiles essentielles de deux Apiacées (Ferula vesceritensis Coss et DR et Balansea glaberrima Desf.) Lange

Abstract

Because of the high level of résistance phenomenon to antibiotics being developed, the discovery of new antibacterial is being more than necessary. In addition, to avoid the con sequences of oxidative Stress, it is necessary to reestablish the equilibrium of the balance oxidant/antioxidant.The screening of essential oils permits the discovery of new antibacterial and antioxidant. Our study contributes to the knowledge of antibacterial and antioxidant properties of essential oils of two aromatic plants of the family Apiacées, namely Ferula vesceritensis and Balansea glaberrima. Essentials oils were extracted by hydrodistillation and their chemical composition was analysed by Gas-Chromatography coupled to Mass-spectrometry (CPG/SM): α–pinéne (28, 57%) and aristolene (24, 63%) were the major compounds of F.vesceritensis, but the valencene (36, 65%),l’apiole (34, 87%) and γ-muroléne (9, 83%) were the major compounds in E.O of B. glaberrima. Antimicrobial activity was determined using the agar diffusion test. The results of the antibacterial activity demonstrated the effect of E.O of F.vesceritensis towards S.aureus ATCC 25923 and B.subtillus ATCC 6633. The E.O of B.glaberrimais not only active towards those two later strains but E. coli ATCC 25922 as well. A.flavusNRRL 391 was inhibited by E.Oil of B.glaberrima.C. albicans ATCC 1024 was lowly inhibited by E.O of F. vesceritensis.The antioxidant activity was evaluated using the free radical scavenging effect of the DPPHE.O of F.vesceritensis and B.glaberrima demonstrated an effect antiradicalar very low in comparison with BHT, the IC50 of B. glaberrima, F.vesceritensis and BHT: 9136,93µg/ml, 7440,68µg/ml, 41,35µg/ml wererespectively.