Extraction et dosage quantitatif de l’ADN par méthode fluorimétrique : application à l’ADN humain et à l’ADN viral

Abstract

DNA extraction is a critical step in molecular biology, determining the reliability of downstream analyses, whether for genetic mutation detection or pathogen identification. This study aimed to evaluate the influence of various factors (sample type, storage conditions, temperature, and storage duration) on the quality and quantity of extracted DNA, both human and viral. DNA extraction was carried out using a commercial spin column kit, followed by fluorometric quantification with the Qubit method. The results showed a wide range of DNA concentrations depending on sample processing conditions, with levels varying from low to high based on preservation, cell type, and storage time. The protocol proved to be sensitive, reproducible, and suitable for molecular biology requirements. The findings highlight the importance of an optimized and rigorous protocol to ensure reliable results, particularly for genetic and virological diagnostics.