Activité anti-inflammatoire, antioxydante et anticoagulante de l’extrait aqueux des feuilles de Parietaria judaica L.

Abstract

This study focuses on the phytochemical analysis and the evaluation of the antiinflammatory, antioxidant, and anticoagulant activities of the aqueous extract of Parietaria judaica leaves. Quantitative analysis revealed that the extract contains 25.14 ± 0.68 µg EAG/mg of total polyphenols and 3.93 ± 0.98 µg QE/mg of flavonoids. The anti-inflammatory activity was assessed in vitro using human erythrocyte membrane stabilization and protein denaturation inhibition assays. The extract demonstrated protective effects on erythrocyte membranes and significantly inhibited protein denaturation, with inhibition rates of 71.52% and 80.24%, respectively. The antioxidant activity was evaluated using ABTS radical scavenging and reducing power assays. Results indicated a moderate free radical scavenging effect, with an IC₅₀ of 443.33 ± 28.52 µg/ml, and a concentration-dependent reducing power with an EC₅₀ of 277.5 µg/ml. Regarding its anticoagulant and antithrombotic properties, the aqueous extract at 50 mg/ml significantly prolonged coagulation time to 22 min 13 s, while at 25 mg/ml, it showed an antithrombotic effect of 38%. In conclusion, the aqueous extract of Parietaria judaica leaves exhibits interesting anti-inflammatory, antioxidant, anticoagulant, and antithrombotic activities, likely due to their polyphenol content, which supports its traditional use