Recherche de l’HPV-HR oncogène par biologie moléculaire dans le cadre du dépistage du cancer du col de l’utérus

Abstract

Cervical cancer is among the most common cancers for women and is closely associated with persistent infection by high-risk human papillomavirus (HPV-HR), particularly genotypes 16 and 18. This study aims to evaluate the effectiveness of molecular biology techniques, particularly real-time PCR, in the early detection of this virus from cervicouterine samples collected as part of a regional screening program. The study involved 38 samples collected from the gynecology department of the University Hospital of Setif and processed at the central laboratory of the CLCC of Setif. Following the extraction of viral DNA, PCR analyses detected three positive cases, including infections by HPV-HR genotypes known for their high oncogenic potential. The results confirm the usefulness of PCR as a sensitive and reliable tool for the early detection of HPV-HR. They support its integration into national cervical cancer prevention strategies, particularly in areas with limited healthcare access.