Étude comparative du dosage de la vitamine d sur cobras e411® versus vidas®.

Abstract

Vitamin D is an essential vitamin for regulating bone metabolism and maintaining healthy bones. Deficiencies are frequent and often unrecognised, which makes measuring this vitamin a valuable indicator for adapting an effective treatment. There are two methods of determination: non-immunological methods and immunological methods, which are the most common. The aim of this study was to compare the 25(OH) D assay between two different devices; Cobas e411® versus Vidas® which have the same assay principle (competitive enzyme-linked immunosorbent assay) and two different detection techniques. We wanted to check whether or not the results provided by one of the devices were comparable with the results obtained by the other. The patients in the study were divided between men (29.1%) and women (70.9%), with an average age of 34. The linear regression plot of Bablok et al shows a good correlation between the two analysers, with the regression equationy = 1.109x + 4.385. The results of the difference diagram according to Bland and Altman show good agreement between the two methods. The concordance rate for patient classification was 92.5% at the recommended international deficiency threshold ofof 30 ng/mL. In order to evaluate the analytical performance of the different analysers and methods, numerous studies comparing 25(OH) D assays have been carried out, some of which have compared the Cobas e411® with other analyzers, while other studies have compared the vidas® with other analyzers. By comparing the results of our study with the previous data, we can confirm the reliability of these two analyzers.