Préparation et caractérisation physico-chimique des nanoparticules à base d’extrait de grenade et évaluation des activités biologique

Abstract

The nanoprecipitation method was used to encapsulate bioactive compounds and enhance their characteristics. This work aims to encapsulate extracts from the yellow peel of pomegranate. The bioactive constituents were extracted and analyzed qualitatively and quantitatively by LC MS, showing that the three extracts are rich in phenolic compounds. In vitro tests for antioxidant, anti-inflammatory and antibacterial activities of the different extracts revealed remarkable antioxidant activity, with notable IC50 values for ABTS (7.05±0.12µg/ml), CUPRAC (7.40±0.79 µg/ml) and GOR (6.03±1.12 µg/mL) for the aqueous extract. In addition, the hydro-methanolic extract showed superior antibacterial efficacy against selected bacterial strains, while for anti-inflammatory activity, all three extracts possessed a potent anti inflammatory effect. These results are confirmed by in silico analyses exploring the interaction between specific compounds and various solvents. The encapsulation results show that gelatin can successfully encapsulate the three pomegranate yellow peel extracts, leading to the formation of nanocapsules. Various characterizations and measurements, including XRD, FTIR, AFM,MEB, Zeta potential, and particle size analysis, confirmed the formation of nanoparticles from the different pomegranate extracts. This study underlines the promising potential of pomegranate skin extract nanoparticles, highlighting their ability to improve stability and solubility.