Evaluation des activités biologiques du décocté de Lavendula stoechas

Abstract

The present work focuses on the phytochemical study and evaluation of anti-inflammatory, antioxidant, anticoagulant and antithrombotic activities of the aqueous extract of Lavandula stoechas. Quantitative analysis showed that the aqueous extract contains 217.48±2.44 µg GAE/mg extract total polyphenols and 1.85±0.082 µg QE/mg extract flavonoids. The antioxidant capacity of the aqueous extract was measured using four tests: DPPH, ABTS hydrogen peroxide scavenging and reducing power. The results obtained show that the extract possesses excellent free radical scavenging activity against DPPH• with an IC₅₀ of 19.16±1.67 µg/mL and ABTS•+ with an IC₅₀ of 184.23±14.31 µg/mL, for the reducing power, the extract exhibits a potent activity with an EC₅₀ of 46.94± 0.487µg/mL. It also shows a scavenging effect of hydrogen peroxide with an IC₅₀ of 52.32±1.30 µg/mL. The antiinflammatory activity was evaluated in vitro using two tests: inhibition of thermal protein denaturation and erythrocyte membrane stabilization. Results show that the aqueous extract (25-500 μg/mL) effectively inhibits protein denaturation with percentages ranging from 40.5% to 92.9%, comparable to diclofenac. The extract also preserves erythrocyte membranes against hemolysis with protection reaching 85.33% at 3 mg/mL, surpassing diclofenac (72.81%) at the same concentration