Etude anti oxydante et anti inflammatoire des extraits de Bunium incrassatum

Abstract

This study aims to evaluate the biological effect of five extracts: methanolic extract (EMeOH), ethyl acetate extract (EAc), aqueous extract (EAq), butanolic extract (EBut), and bark extract (EEc). These extracts were obtained by maceration of powdered tubers of the plant Bunium incrassatum. The quantitative estimation of the content of these extracts in polyphenols and flavonoids showed that EAc has the highest content (47.08±2.64 mg GAE/mgE and 5.26±1.5 mg QE/mgE respectively). The evaluation of antioxidant activity by the hydrogen peroxide (H2O2) scavenging test showed that EAq is the most active (17.20±1.84 µg EAA/mgE). The anti-inflammatory activity using the model, Croton oil-induced ear edema, showed that EMeOH at doses of 150 mg/kg and 300 mg/kg exhibited an inhibition percentage (53.33% and 64.44% respectively) as significant as that obtained in the presence of indomethacin (ns: p > 0.05). On the other hand, the contribution of lipid peroxidation inhibition to antioxidant activity was evaluated by measuring the level of MDA (malondialdehyde) and nitric oxide (NO·) production. The results showed that the EMeOH extract at the dose of 300 mg/kg exhibited a significant effect (106.15 ± 5.33 nmole/g of tissue) compared to the group treated with vitamin C (143.46 ± 2.72 nmole/g of tissue). The nitrite content obtained for both doses of 150 mg/kg and 300 mg/kg of extract (9.58 ± 0.32µM and 10.58 ± 1.73 µM respectively) did not show a significant difference compared to that of the C+ group. These results indicate that the plant Bunium incrassatum constitutes a promising source of antioxidant and anti-inflammatory