Evaluation des activités antioxydantes, anti-hémolytiques et Anti-inflammatoires de deux extraits des feuilles de Cupressus sempervirens

Abstract

The objective of this study is to evaluate the antioxidant, anti-hemolytic, and anti-inflammatory activity of the methanolic extract (E. Met) and ethyl acetate extract (AE) from the leaves of Cupressus sempervirens L. Extraction by maceration in methanol yielded 28.6% for E. Met and 8.61% for AE obtained by fractionation of E. Met. Phytochemical analysis revealed that E. Met was richer in polyphenols with a content of 141.37±28.66 mg GAE/g, while AE was richer in flavonoids with a content of 37.16 ± 1.36 mg QE/g of dry extract. Antioxidant activity was evaluated using DPPH, hydrogen peroxide (H₂O₂), and nitric oxide (NO) scavenging assays. AE showed the highest antioxidant activity with an IC50 = 0.011 ± 0.001 mg/ml for DPPH scavenging, an IC50 = 0.0865 ± 0.0113 mg/ml for H₂O₂ scavenging, and inhibition percentages of 84% and 82.5% for AE and E. Met respectively in the NO scavenging test. Regarding the APPH-induced hemolysis test, AE showed an HT50 of 105 minutes. Anti-inflammatory activity was evaluated by two in vitro tests : 1) The BSA denaturation test, in which AE showed the highest inhibitory activity with an inhibition rate of 46.91%. 2) For the membrane stability test, AE was also the most effective with an inhibition percentage of 81.35%. This study demonstrated that the leaves of Cupressus sempervirens L. have remarkable antioxidant, antihemolytic, and anti-inflammatory activities, which supports their traditional use.