Synthesis, Characterization and Biological Activities of New Thiocarbohydrazide Schiff bases Derivatives

Abstract

This work focuses on the synthesis, characterization, and biological evaluation of three novel asymmetric thiocarbohydrazide-based Schiff base derivatives (TCHB, TCHD, and TCHF). Synthesized via the condensation of TCH with benzoin, dehydroacetic acid, or furoin, these compounds were isolated with satisfactory yields ranging from 32.5% to 39.5%. Their structures were confirmed by physicochemical and spectroscopic analyses (FT-IR, UV-Visible), validating the presence of azomethine, thiocarbonyl, hydroxyl, and carbonyl functional groups. In vitro biological evaluation revealed a selective activity profile: compound TCHF demonstrated potent antioxidant activity (ABTS radical scavenging, IC₅₀ = 7.18 μM), whereas no significant anticholinesterase activity (BChE inhibition) was observed for any of the tested molecules