Évaluation de l’activité antioxydante, anti-inflammatoire et anticoagulante de la plante médicinale algérienne.

Abstract

Oxidative stress is the cause of a number of diseases, is driving the search for new antioxidant remedies. Galium aparine L is a widespread plant in temperate zones, commonly known as bedstraw, belonging to the Rubiaceae family and traditionally used for its medicinal applications. The aim of the present study was to investigate the antioxidant, anticoagulant and anti-inflammatory activities following quantification of bioactive compounds, in particular polyphenols, flavonoids and tannins from aqueous and hydroethanolic extracts of Galium aparine L leaves and stems harvested in the Beni Ourthilan region, wilaya of Sétif. In vitro antioxidant activity was estimated using four different tests: DPPH, ferrous iron chelation, reducing power and β-carotene. Quantitative estimation of total phenolic compounds using the spectrophotometric method showed that the aqueous extract is rich in polyphenols, unlike flavonoids and tannins, which are more abundant in the hydroethanol extract. This study showed that the aqueous extract has excellent antioxidant power compared to the hydroethanolic extract in most tests, particularly the chelation of ferrous ions, which showed an IC50 value of 0.048 ± 0.0008 mg/m