Phytochimie et activité anti-oxydante et anti-inflammatoire de l’extrait de Silene colorata L.

Abstract

Silene colorata is a medicinal plant belonging to the Caryophyllaceae family and is widely distributed in certain Mediterranean regions. This species is known for its richness in secondary metabolites of significant phytochemical and pharmacological interest. The objective of this study was to determine the extraction yield of the 70% hydroethanolic extract obtained by maceration from the aerial parts of the plant, to identify and quantify certain secondary metabolites, and to evaluate its antioxidant and anti-inflammatory activities. The extraction yield obtained was 8.26% (w/w). Phytochemical screening revealed the presence of polyphenols and tannins, as well as traces of quinons and anthraquinons, while flavonoids, saponins, and terpenoids were absent. The total polyphenol content, determined by the Folin–Ciocalteu method, was 10.63 ± 0.021 µg GAE/mg DE. The determination of total flavonoids using the aluminum chloride method revealed a content of 3.95 ± 0.04 µg QE/mg DE, whereas the flavone and flavonol content reached 14.87 ± 0.11 µg QE/mg DE. Condensed tannins quantified by the vanillin method showed a value of 49.38 ± 3.46 µg CE/mg DE. The antioxidant activity was evaluated using different tests, free radical scavenging DPPH, ferric reducing power FRAP, and total antioxidant capacity TAC. The DPPH free radical scavenging test showed an activity expressed as IC50 of 306 ± 12.75 µg/ml. The FRAP assay revealed a reducing power expressed as EC50 of 4700 ± 0.035 µg/ml. Regarding the phosphomolybdenum TAC assay, the extract exhibited percentages of 80.07% and 97.91% using ascorbic acid and quercetin as standards, respectively. The in vitro anti-inflammatory activity also demonstrated a significant biological effect 546,97±5,79µg/ml knowing that aspirin, used as a standard, showed an activity of 389,78±12,92 µg/ml. These results endorse the interest showed for this plant and then its use as a medicinal plant.

Keywords: Caryophyllaceae, Silene colorata, maceration, hydro-ethanolic extract, secondary metabolites, antioxidante activity, anti-inflammatory activity