Identification des enzymes clés impliquée dans la biodégradation de la matière organique de Bacillus sp1 et Pseudomonas sp1.

Abstract

In this work, two bacterial strains, Bacillus sp1 (M 8.1) and Pseudomonas sp1 (M 20.3), isolated from aquatic soil samples, were evaluated for their ability to produce four ecological relevant enzymes: amylase, protease, cellulase, and chitinase. Enzymatic assays revealed that Pseudomonas sp1 exhibited notable amylolytic activity, with an average hydrolysis zone of 4.3 cm, surpassing that of Bacillus sp1 2.15 cm . A similar trend was observed for protease activity, with average halo diameters of 1.33 cm for Pseudomonas sp1 compared to 0.7 cm for Bacillus sp1, indicating a higher enzymatic potential in the former. As for cellulase activity, Pseudomonas sp1 also showed better results, with an average degradation zone of 2.2 cm, compared to 1.95 cm for Bacillus sp1.Regarding chitinase activity, the same trend was recorded2.85 cm for Pseudomonas sp1 versus 2.35 cm for Bacillus sp1, further confirming its high enzymatic activity.These findings highlight the promising biotechnological potential of Pseudomonas sp1, particularly for industrial applications requiring hydrolytic enzymes, although Bacillus remains a classical reference in the field.