Docking et étude de la prévalence des souches bactériennes cliniques résistantes aux aminoglycosides dans la région de Sétif

Abstract

Antibiotic resistance is a growing global challenge, complicating the treatment of infections. Aminoglycosides, although effective against serious infections, are also implicated, increasing the risk of complications and mortality. This study aimed to analyze the epidemiological profile of 135 aminoglycoside-resistant strains in Setif between 2019 and 2021, and to study using molecular docking, their interactions with the enzymes responsible for this resistance. Antibiograms were performed on 135 bacterial strains according to CLSI standards. Phenotypic tests assessed the virulence of the strains, particularly through biofilm and enzyme production. Statistical analysis was conducted using SPSS. The epidemiological results showed a predominance of resistance in women (55.6%), with a majority of cases in adults (68.1%). Clinically, urinary tract infections were the most common (76.3%), and bacteriologically, Escherichia coli was the most frequently isolated bacterium (40.7%). Phenotypic testing revealed high resistance to several antibiotics, including amoxicillin (83%), amoxicillin-clavulanic acid (80%), cephalexin (74.8%), cefixime (71.1%), trimethoprim-sulfamethoxazole (74.8%), and gentamicin (72.6%). Chloramphenicol (53.3%), imipenem (47.4%), amikacin (47.4%), and piperacillin-tazobactam (31.1%) exhibited relatively high susceptibility rates.