Evaluation de l’activité antioxydante et anticoagulante par décoction de Verbascum thapsus

Abstract

The objective of this study is to evaluate the antioxidant and anticoagulant activities of the aqueous extract from the aerial part of the Verbascum thapsus. The antioxidant activity is determined by the DPPH• and ABTS•+ radical scavenging tests, the hydrogen peroxide (H2O2) test and the reducing power. The results obtained show that the extraction yield by decoction is 22,95% and that the content of the extract in polyphenols and flavonoids is 72,93 µg EAG/mg and 47,10 µg EQ/mg, respectively. The evaluation of anti-radical activity by DPPH• and ABTS•+ showed that the extract has an anti-radical activity lower than the standards BHT and BHA, with IC50 values of 35,39±2,23 µg/mL and 9,73±0,46 µg/mL, respectively. In the H2O2 neutralization test, the extract showed an effect lower than the standard with an IC50 of 53,65±2,85 µg/mL compared to vitamin C 36,49±0,74 µg/mL. The extract exhibited a reducing effect with an EC50 of 36,44±0,93 µg/mL, which is lower than BHT (14,55±1,33 µg/mL). Finally the extract had no effect in anticoagulant and antithrombotic activities tests. In conclusion, the aqueous extract of Verbascum thapsus possesses remarkable antioxidant activity, possibly due to the presence of phenolics compounds. Such results can be exploited in the food and pharmaceutical industries